Serum Protein Concentration and Serum Protein Fractions in Bottlenose Dolphins (Tursiops truncatus) under Human Care Using Agarose Gel Electrophoresis

Serum protein electrophoresis (SPE) is the most used and reliable method to determine percentage of serum subfractions. The interpretation kinetics total proteins albumin globulin fractions receiving increased attention in wild animals, as well domestic due possibility identifying typical pathologic patterns. However, these data had be performed light an appropriate method—and species- specific reference intervals (RIs). In marine mammals, other non-domestic species, should also given different environment (free ranging vs. human managed) associated exposure environmental stimuli. aim this report was establish RIs for evaluated using agarose gel (AGE) bottlenose dolphins under care. Peripheral blood samples were collected from 40 during standard veterinary procedures evaluate their health status. Total concentration determined biuret while AGE automated system. A pooled dolphin’s sample intra-assay inter-assay imprecision AGE. calculated Excel spreadsheet with Reference Value Advisor set macroinstructions. intra imprecisions 1.2% 2.5%, respectively, albumin; 2.9% 5.7%, α-globulins; 3.8% 4.0%, β-globulins; 3.4% 4.8%, γ-globulins. protein, albumin, α-globulin, β-globulin, γ-globulin concentrations 65.5 ± 5.4 g/L, 45.5 4.9 8.0 1.0 5.0 2.0 7.0 respectively. We established